This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DPH5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DPH5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DPH5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:100,833,854–100,835,941 | 190.5 kb | Distal (>10kb) Multiome | 227 | |
| chr1:100,894,532–100,896,945 | 129.7 kb | Distal (>10kb) Multiome | 955 | |
| chr1:100,927,261–100,928,428 | 97.8 kb | Distal (>10kb) Multiome | 102 | |
| chr1:101,025,253–101,026,441 | 147 bp | At TSS Multiome | 842 | |
| chr1:101,235,386–101,237,733 | 211.0 kb | Distal (>10kb) Multiome HiCAR | 598 | |
| chr1:101,308,998–101,310,788 | 284.0 kb | Distal (>10kb) Multiome | 506 |
Genomic view of the DPH5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.