This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for DPH2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DPH2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DPH2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:43,707,108–43,708,162 | 262.4 kb | Distal (>10kb) Multiome | 937 | |
| chr1:43,933,385–43,935,511 | 36.3 kb | Distal (>10kb) Multiome | 565 | |
| chr1:43,935,659–43,937,001 | 33.3 kb | Distal (>10kb) Multiome | 359 | |
| chr1:43,946,199–43,947,337 | 23.3 kb | Distal (>10kb) Multiome | 1003 | |
| chr1:43,969,717–43,970,284 | 76 bp | At TSS Multiome | 515 | |
| chr1:43,974,246–43,975,571 | 4.8 kb | Proximal (<10kb) Multiome | 875 | |
| chr1:43,978,661–43,979,381 | 9.0 kb | Proximal (<10kb) Multiome | 419 | |
| chr1:43,991,242–43,992,448 | 21.9 kb | Distal (>10kb) Multiome | 780 | |
| chr1:44,029,597–44,032,070 | 61.7 kb | Distal (>10kb) Multiome | 896 | |
| chr1:44,213,251–44,213,777 | 243.5 kb | Distal (>10kb) Multiome | 1062 | |
| chr1:44,238,024–44,238,695 | 268.4 kb | Distal (>10kb) Multiome | 396 |
Genomic view of the DPH2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.