The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for DOT1L. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DOT1L upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DOT1L, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:1,861,439–1,865,723 | 300.0 kb | Distal (>10kb) Multiome | 891 | |
| chr19:1,875,645–1,876,693 | 287.7 kb | Distal (>10kb) Multiome | 887 | |
| chr19:1,885,058–1,885,861 | 278.4 kb | Distal (>10kb) Multiome | 495 | |
| chr19:1,904,556–1,906,084 | 258.5 kb | Distal (>10kb) Multiome | 907 | |
| chr19:1,940,240–1,942,987 | 222.9 kb | Distal (>10kb) Multiome | 901 | |
| chr19:2,034,637–2,035,967 | 128.9 kb | Distal (>10kb) Multiome | 368 | |
| chr19:2,041,744–2,042,811 | 121.7 kb | Distal (>10kb) Multiome | 586 | |
| chr19:2,049,171–2,052,365 | 112.6 kb | Distal (>10kb) Multiome | 977 | |
| chr19:2,060,703–2,062,560 | 102.4 kb | Distal (>10kb) Multiome | 913 | |
| chr19:2,095,898–2,097,954 | 67.2 kb | Distal (>10kb) Multiome | 945 | |
| chr19:2,150,726–2,152,200 | 12.2 kb | Distal (>10kb) Multiome | 761 | |
| chr19:2,163,561–2,164,587 | 59 bp | At TSS Multiome | 750 | |
| chr19:2,165,086–2,165,623 | 1.2 kb | Proximal (<10kb) | 552 | |
| chr19:2,235,315–2,237,253 | 72.7 kb | Distal (>10kb) Multiome | 1119 | |
| chr19:2,269,007–2,271,589 | 105.5 kb | Distal (>10kb) Multiome | 983 | |
| chr19:2,273,467–2,274,136 | 109.9 kb | Distal (>10kb) Multiome | 825 | |
| chr19:2,327,828–2,329,150 | 164.7 kb | Distal (>10kb) Multiome | 759 | |
| chr19:2,424,712–2,426,099 | 261.4 kb | Distal (>10kb) Multiome | 261 | |
| chr19:2,426,254–2,428,729 | 263.8 kb | Distal (>10kb) Multiome | 1035 | |
| chr19:2,456,419–2,457,381 | 293.1 kb | Distal (>10kb) Multiome | 579 | |
| chr19:2,461,902–2,462,483 | 298.1 kb | Distal (>10kb) Multiome | 382 | |
| chr19:2,462,877–2,463,379 | 299.3 kb | Distal (>10kb) Multiome | 764 |
Genomic view of the DOT1L locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.