This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Transcription factors with Perturb-seq knockdown data for DLD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DLD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DLD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:107,660,462–107,662,091 | 229.6 kb | Distal (>10kb) Multiome | 578 | |
| chr7:107,742,953–107,744,685 | 147.3 kb | Distal (>10kb) Multiome | 1055 | |
| chr7:107,890,620–107,891,827 | 61 bp | At TSS Multiome | 950 | |
| chr7:107,932,723–107,933,235 | 41.8 kb | Distal (>10kb) Multiome | 109 | |
| chr7:108,001,297–108,001,784 | 110.3 kb | Distal (>10kb) Multiome | 244 | |
| chr7:108,001,968–108,003,797 | 112.0 kb | Distal (>10kb) Multiome | 685 | |
| chr7:108,134,914–108,135,839 | 244.1 kb | Distal (>10kb) Multiome | 247 |
Genomic view of the DLD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.