This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Transcription factors with Perturb-seq knockdown data for DHX8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DHX8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DHX8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:43,211,494–43,212,392 | 272.0 kb | Distal (>10kb) Multiome | 586 | |
| chr17:43,359,784–43,361,254 | 122.9 kb | Distal (>10kb) Multiome | 981 | |
| chr17:43,367,132–43,369,458 | 114.8 kb | Distal (>10kb) Multiome | 1146 | |
| chr17:43,388,101–43,389,658 | 94.6 kb | Distal (>10kb) Multiome HiCAR | 770 | |
| chr17:43,396,555–43,396,987 | 87.2 kb | Distal (>10kb) Multiome HiCAR | 27 | |
| chr17:43,398,359–43,400,469 | 85.1 kb | Distal (>10kb) Multiome HiCAR | 827 | |
| chr17:43,403,154–43,404,714 | 80.0 kb | Distal (>10kb) Multiome HiCAR | 252 | |
| chr17:43,406,748–43,407,604 | 76.8 kb | Distal (>10kb) Multiome HiCAR | 102 | |
| chr17:43,438,695–43,439,408 | 45.0 kb | Distal (>10kb) Multiome | 86 | |
| chr17:43,457,977–43,459,063 | 25.5 kb | Distal (>10kb) Multiome | 328 | |
| chr17:43,483,594–43,484,268 | 113 bp | At TSS Multiome | 1016 | |
| chr17:43,530,368–43,531,356 | 46.9 kb | Distal (>10kb) Multiome | 828 | |
| chr17:43,545,066–43,547,010 | 61.7 kb | Distal (>10kb) Multiome | 999 | |
| chr17:43,694,216–43,694,699 | 210.5 kb | Distal (>10kb) Multiome | 408 | |
| chr17:43,720,542–43,721,150 | 236.8 kb | Distal (>10kb) Multiome | 572 | |
| chr17:43,755,308–43,755,795 | 271.5 kb | Distal (>10kb) Multiome | 248 | |
| chr17:43,760,231–43,760,686 | 276.5 kb | Distal (>10kb) Multiome | 539 | |
| chr17:43,778,079–43,779,460 | 294.9 kb | Distal (>10kb) Multiome | 937 |
Genomic view of the DHX8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.