DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]
Transcription factors with Perturb-seq knockdown data for DHX30. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DHX30 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DHX30, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:47,512,910–47,514,044 | 289.6 kb | Distal (>10kb) Multiome HiCAR | 892 | |
| chr3:47,522,175–47,522,702 | 280.8 kb | Distal (>10kb) Multiome HiCAR | 591 | |
| chr3:47,577,493–47,579,738 | 223.8 kb | Distal (>10kb) Multiome | 446 | |
| chr3:47,781,220–47,782,492 | 21.2 kb | Distal (>10kb) Multiome | 965 | |
| chr3:47,802,571–47,803,701 | 17 bp | At TSS Multiome | 820 | |
| chr3:47,824,603–47,825,589 | 21.8 kb | Distal (>10kb) Multiome | 882 | |
| chr3:48,088,196–48,089,485 | 285.9 kb | Distal (>10kb) Multiome | 933 |
Genomic view of the DHX30 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.