The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DHODH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DHODH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DHODH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:71,722,829–71,724,442 | 285.6 kb | Distal (>10kb) Multiome | 894 | |
| chr16:71,808,251–71,809,603 | 199.6 kb | Distal (>10kb) Multiome | 1032 | |
| chr16:71,845,428–71,846,392 | 162.8 kb | Distal (>10kb) Multiome | 923 | |
| chr16:71,883,009–71,884,742 | 124.6 kb | Distal (>10kb) Multiome | 791 | |
| chr16:71,894,288–71,896,022 | 113.4 kb | Distal (>10kb) Multiome | 1139 | |
| chr16:71,981,752–71,982,390 | 26.7 kb | Distal (>10kb) Multiome | 263 | |
| chr16:72,008,426–72,009,172 | 107 bp | At TSS Multiome | 815 | |
| chr16:72,013,585–72,014,228 | 5.3 kb | Proximal (<10kb) Multiome | 263 | |
| chr16:72,093,288–72,094,355 | 85.0 kb | Distal (>10kb) Multiome | 860 | |
| chr16:72,111,833–72,112,319 | 103.4 kb | Distal (>10kb) Multiome | 60 | |
| chr16:72,202,429–72,203,074 | 194.0 kb | Distal (>10kb) Multiome | 160 |
Genomic view of the DHODH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.