Predicted to enable zinc ion binding activity. Predicted to be involved in lysosome localization; positive regulation of bone resorption; and positive regulation of ruffle assembly. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for DEF8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DEF8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DEF8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:89,657,432–89,658,521 | 291.0 kb | Distal (>10kb) Multiome | 859 | |
| chr16:89,686,359–89,687,641 | 262.0 kb | Distal (>10kb) Multiome | 1006 | |
| chr16:89,699,933–89,702,566 | 246.8 kb | Distal (>10kb) Multiome | 1079 | |
| chr16:89,711,278–89,712,334 | 237.0 kb | Distal (>10kb) Multiome | 443 | |
| chr16:89,720,440–89,722,486 | 227.4 kb | Distal (>10kb) Multiome | 1048 | |
| chr16:89,816,022–89,817,100 | 132.1 kb | Distal (>10kb) Multiome | 995 | |
| chr16:89,827,667–89,829,190 | 120.3 kb | Distal (>10kb) Multiome | 784 | |
| chr16:89,872,618–89,874,723 | 74.6 kb | Distal (>10kb) Multiome | 1046 | |
| chr16:89,917,809–89,919,023 | 30.2 kb | Distal (>10kb) Multiome | 986 | |
| chr16:89,921,530–89,925,204 | 25.6 kb | Distal (>10kb) Multiome | 1006 | |
| chr16:89,925,437–89,926,738 | 22.5 kb | Distal (>10kb) Multiome | 654 | |
| chr16:89,948,051–89,948,388 | 401 bp | At TSS | 502 | |
| chr16:89,948,487–89,948,865 | at TSS | At TSS | 721 | |
| chr16:89,958,035–89,958,351 | 9.2 kb | Proximal (<10kb) | 78 | |
| chr16:89,972,039–89,973,313 | 23.9 kb | Distal (>10kb) Multiome | 965 | |
| chr16:90,018,651–90,020,274 | 70.9 kb | Distal (>10kb) Multiome | 634 | |
| chr16:90,046,432–90,047,827 | 98.1 kb | Distal (>10kb) Multiome | 700 | |
| chr16:90,077,247–90,078,026 | 128.7 kb | Distal (>10kb) Multiome | 412 | |
| chr16:90,081,997–90,082,775 | 133.4 kb | Distal (>10kb) Multiome | 755 |
Genomic view of the DEF8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.