This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for DCTN3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DCTN3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DCTN3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:34,328,711–34,329,961 | 291.1 kb | Distal (>10kb) Multiome | 768 | |
| chr9:34,363,597–34,364,212 | 256.6 kb | Distal (>10kb) Multiome | 104 | |
| chr9:34,374,953–34,375,681 | 245.3 kb | Distal (>10kb) Multiome | 432 | |
| chr9:34,376,008–34,377,655 | 243.5 kb | Distal (>10kb) Multiome | 779 | |
| chr9:34,379,248–34,381,674 | 239.6 kb | Distal (>10kb) Multiome | 763 | |
| chr9:34,456,881–34,459,235 | 161.9 kb | Distal (>10kb) Multiome | 704 | |
| chr9:34,522,211–34,523,972 | 97.3 kb | Distal (>10kb) Multiome | 473 | |
| chr9:34,577,374–34,578,752 | 42.5 kb | Distal (>10kb) Multiome | 204 | |
| chr9:34,589,298–34,591,068 | 30.7 kb | Distal (>10kb) Multiome | 258 | |
| chr9:34,591,283–34,592,013 | 28.7 kb | Distal (>10kb) Multiome | 368 | |
| chr9:34,611,663–34,612,738 | 8.4 kb | Proximal (<10kb) Multiome | 680 | |
| chr9:34,612,834–34,613,263 | 7.4 kb | Proximal (<10kb) Multiome HiCAR | 343 | |
| chr9:34,620,108–34,620,704 | 17 bp | At TSS Multiome | 467 | |
| chr9:34,628,770–34,629,308 | 8.5 kb | Proximal (<10kb) Multiome | 216 | |
| chr9:34,637,123–34,638,475 | 17.3 kb | Distal (>10kb) Multiome HiCAR | 872 | |
| chr9:34,646,247–34,647,338 | 26.2 kb | Distal (>10kb) Multiome | 857 | |
| chr9:34,651,771–34,652,431 | 31.6 kb | Distal (>10kb) Multiome | 669 | |
| chr9:34,664,346–34,666,446 | 45.0 kb | Distal (>10kb) Multiome | 707 | |
| chr9:34,674,895–34,675,574 | 54.7 kb | Distal (>10kb) Multiome | 372 | |
| chr9:34,701,127–34,701,863 | 80.9 kb | Distal (>10kb) Multiome | 390 |
Genomic view of the DCTN3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.