This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2012]
Transcription factors with Perturb-seq knockdown data for DCTN2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DCTN2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DCTN2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:56,629,725–56,630,674 | 916.9 kb | Distal (>10kb) Multiome HiCAR | 759 | |
| chr12:56,633,838–56,637,505 | 912.7 kb | Distal (>10kb) Multiome HiCAR | 1037 | |
| chr12:57,270,778–57,271,413 | 276.0 kb | Distal (>10kb) Multiome | 155 | |
| chr12:57,272,508–57,273,156 | 274.3 kb | Distal (>10kb) Multiome | 98 | |
| chr12:57,377,252–57,378,039 | 169.5 kb | Distal (>10kb) Multiome | 170 | |
| chr12:57,430,384–57,431,415 | 116.2 kb | Distal (>10kb) Multiome | 879 | |
| chr12:57,454,516–57,456,639 | 90.8 kb | Distal (>10kb) Multiome | 841 | |
| chr12:57,458,743–57,463,318 | 85.8 kb | Distal (>10kb) Multiome | 994 | |
| chr12:57,475,229–57,476,151 | 71.6 kb | Distal (>10kb) Multiome | 194 | |
| chr12:57,487,577–57,488,336 | 59.2 kb | Distal (>10kb) Multiome | 784 | |
| chr12:57,519,488–57,521,440 | 26.5 kb | Distal (>10kb) Multiome HiCAR | 1029 | |
| chr12:57,521,541–57,523,658 | 24.5 kb | Distal (>10kb) Multiome HiCAR | 960 | |
| chr12:57,546,771–57,547,419 | 64 bp | At TSS Multiome | 623 | |
| chr12:57,549,654–57,550,343 | 2.9 kb | Proximal (<10kb) Multiome | 366 | |
| chr12:57,583,383–57,583,948 | 36.4 kb | Distal (>10kb) Multiome | 353 | |
| chr12:57,590,813–57,591,877 | 44.0 kb | Distal (>10kb) Multiome | 738 | |
| chr12:57,604,466–57,605,404 | 57.7 kb | Distal (>10kb) Multiome | 337 | |
| chr12:57,609,052–57,609,957 | 62.2 kb | Distal (>10kb) Multiome | 83 | |
| chr12:57,610,841–57,611,876 | 64.3 kb | Distal (>10kb) Multiome | 558 | |
| chr12:57,621,603–57,622,199 | 74.7 kb | Distal (>10kb) Multiome | 446 | |
| chr12:57,631,837–57,633,369 | 86.0 kb | Distal (>10kb) Multiome | 706 | |
| chr12:57,693,486–57,694,647 | 146.8 kb | Distal (>10kb) Multiome | 757 | |
| chr12:57,726,079–57,726,879 | 179.1 kb | Distal (>10kb) Multiome | 597 | |
| chr12:57,738,612–57,739,176 | 191.9 kb | Distal (>10kb) Multiome | 201 | |
| chr12:57,744,434–57,745,735 | 197.8 kb | Distal (>10kb) Multiome | 822 | |
| chr12:57,751,806–57,753,147 | 205.3 kb | Distal (>10kb) Multiome | 1030 | |
| chr12:57,754,455–57,756,188 | 207.8 kb | Distal (>10kb) Multiome | 616 | |
| chr12:57,756,649–57,757,193 | 209.8 kb | Distal (>10kb) Multiome | 315 | |
| chr12:57,765,020–57,765,720 | 218.2 kb | Distal (>10kb) Multiome | 472 | |
| chr12:57,766,452–57,767,514 | 219.9 kb | Distal (>10kb) Multiome | 330 | |
| chr12:57,771,528–57,773,018 | 225.0 kb | Distal (>10kb) Multiome | 1006 | |
| chr12:57,782,579–57,783,204 | 235.6 kb | Distal (>10kb) Multiome | 916 | |
| chr12:57,814,980–57,815,520 | 268.2 kb | Distal (>10kb) Multiome | 117 | |
| chr12:57,844,422–57,847,797 | 299.9 kb | Distal (>10kb) Multiome | 1032 |
Genomic view of the DCTN2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.