The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for DCTD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DCTD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DCTD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:182,671,028–182,672,805 | 245.8 kb | Distal (>10kb) Multiome | 207 | |
| chr4:182,715,649–182,716,704 | 201.0 kb | Distal (>10kb) Multiome | 115 | |
| chr4:182,806,223–182,807,324 | 110.8 kb | Distal (>10kb) Multiome | 477 | |
| chr4:182,916,637–182,918,352 | 28 bp | At TSS Multiome | 800 | |
| chr4:182,925,182–182,925,655 | 8.1 kb | Proximal (<10kb) Multiome | 389 | |
| chr4:183,008,425–183,009,297 | 91.4 kb | Distal (>10kb) Multiome | 112 | |
| chr4:183,075,620–183,076,390 | 158.5 kb | Distal (>10kb) Multiome | 86 | |
| chr4:183,097,228–183,100,333 | 181.8 kb | Distal (>10kb) Multiome | 964 | |
| chr4:183,182,941–183,183,813 | 266.0 kb | Distal (>10kb) Multiome | 555 | |
| chr4:183,398,351–183,399,038 | 481.4 kb | Distal (>10kb) Multiome HiCAR | 567 |
Genomic view of the DCTD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.