This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Transcription factors with Perturb-seq knockdown data for DARS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DARS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DARS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:135,741,176–135,742,744 | 243.9 kb | Distal (>10kb) Multiome | 978 | |
| chr2:135,875,648–135,876,919 | 109.2 kb | Distal (>10kb) Multiome | 849 | |
| chr2:135,984,898–135,986,233 | 6 bp | At TSS Multiome | 1057 | |
| chr2:135,997,201–135,997,833 | 11.9 kb | Distal (>10kb) Multiome | 160 | |
| chr2:136,025,017–136,026,218 | 40.0 kb | Distal (>10kb) Multiome | 322 | |
| chr2:136,116,129–136,119,923 | 133.8 kb | Distal (>10kb) Multiome | 623 | |
| chr2:136,125,768–136,126,914 | 140.7 kb | Distal (>10kb) Multiome | 196 | |
| chr2:136,178,675–136,180,367 | 194.0 kb | Distal (>10kb) Multiome | 287 |
Genomic view of the DARS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.