NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5A; MIM 613218) and CYB5 reductase (CYB5R3; MIM 613213) (Zhu et al., 1999 [PubMed 10611283]).[supplied by OMIM, Jan 2010]
Transcription factors with Perturb-seq knockdown data for CYB5R4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CYB5R4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CYB5R4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:83,707,656–83,709,786 | 150.6 kb | Distal (>10kb) Multiome | 496 | |
| chr6:83,852,941–83,854,471 | 6.2 kb | Proximal (<10kb) Multiome | 497 | |
| chr6:83,859,075–83,860,410 | 25 bp | At TSS Multiome | 1062 | |
| chr6:84,033,204–84,034,688 | 174.1 kb | Distal (>10kb) Multiome | 473 | |
| chr6:84,052,161–84,053,196 | 193.0 kb | Distal (>10kb) Multiome | 417 | |
| chr6:84,086,644–84,088,326 | 227.6 kb | Distal (>10kb) Multiome | 191 | |
| chr6:84,139,828–84,140,912 | 280.6 kb | Distal (>10kb) Multiome | 99 | |
| chr6:84,227,112–84,228,449 | 368.0 kb | Distal (>10kb) Multiome HiCAR | 827 | |
| chr6:84,762,310–84,765,235 | 905.0 kb | Distal (>10kb) Multiome HiCAR | 714 | |
| chr6:84,772,862–84,775,261 | 915.2 kb | Distal (>10kb) Multiome HiCAR | 587 |
Genomic view of the CYB5R4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.