This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]
Transcription factors with Perturb-seq knockdown data for CTSA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CTSA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CTSA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:45,764,655–45,765,666 | 126.2 kb | Distal (>10kb) Multiome | 192 | |
| chr20:45,770,709–45,771,657 | 120.1 kb | Distal (>10kb) Multiome | 436 | |
| chr20:45,791,633–45,792,259 | 99.4 kb | Distal (>10kb) Multiome | 882 | |
| chr20:45,812,087–45,813,052 | 78.8 kb | Distal (>10kb) Multiome | 695 | |
| chr20:45,833,493–45,834,622 | 57.4 kb | Distal (>10kb) Multiome | 833 | |
| chr20:45,856,871–45,858,137 | 33.8 kb | Distal (>10kb) Multiome | 884 | |
| chr20:45,880,796–45,881,524 | 10.2 kb | Distal (>10kb) Multiome | 738 | |
| chr20:45,890,127–45,891,800 | 54 bp | At TSS Multiome | 1095 | |
| chr20:45,910,538–45,912,397 | 21.0 kb | Distal (>10kb) Multiome | 713 | |
| chr20:45,933,591–45,935,780 | 43.8 kb | Distal (>10kb) Multiome | 1195 | |
| chr20:45,971,553–45,972,790 | 80.9 kb | Distal (>10kb) Multiome | 1206 | |
| chr20:46,004,647–46,005,315 | 113.7 kb | Distal (>10kb) Multiome | 207 | |
| chr20:46,011,604–46,012,166 | 120.6 kb | Distal (>10kb) Multiome | 239 | |
| chr20:46,013,565–46,014,586 | 122.8 kb | Distal (>10kb) Multiome | 735 | |
| chr20:46,021,209–46,023,166 | 130.7 kb | Distal (>10kb) Multiome | 679 | |
| chr20:46,031,750–46,032,261 | 140.7 kb | Distal (>10kb) Multiome | 227 | |
| chr20:46,058,408–46,058,983 | 167.5 kb | Distal (>10kb) Multiome | 123 | |
| chr20:46,089,092–46,090,355 | 198.6 kb | Distal (>10kb) Multiome | 1047 | |
| chr20:46,091,736–46,092,300 | 200.9 kb | Distal (>10kb) Multiome | 38 | |
| chr20:46,117,897–46,118,755 | 227.0 kb | Distal (>10kb) Multiome | 376 | |
| chr20:46,174,457–46,175,011 | 283.4 kb | Distal (>10kb) Multiome | 277 |
Genomic view of the CTSA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.