This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009]
Transcription factors with Perturb-seq knockdown data for CROT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CROT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CROT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:87,058,811–87,060,346 | 285.9 kb | Distal (>10kb) Multiome | 675 | |
| chr7:87,128,891–87,130,142 | 216.4 kb | Distal (>10kb) Multiome | 290 | |
| chr7:87,151,812–87,153,325 | 193.2 kb | Distal (>10kb) Multiome | 1045 | |
| chr7:87,218,879–87,221,122 | 125.2 kb | Distal (>10kb) Multiome | 908 | |
| chr7:87,344,909–87,346,229 | 130 bp | At TSS Multiome | 838 | |
| chr7:87,464,851–87,465,792 | 119.7 kb | Distal (>10kb) Multiome | 440 | |
| chr7:87,506,649–87,507,949 | 161.8 kb | Distal (>10kb) Multiome | 153 | |
| chr7:87,600,126–87,601,238 | 255.0 kb | Distal (>10kb) Multiome | 659 | |
| chr7:87,627,436–87,629,300 | 282.6 kb | Distal (>10kb) Multiome | 723 |
Genomic view of the CROT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.