This gene encodes a member of a family of proteins that are components of the Crumbs cell polarity complex. In mammals, members of this family are thought to play a role in many cellular processes in early embryonic development. A similar protein in Drosophila determines apicobasal polarity in embryonic epithelial cells. Mutations in this gene are associated with focal segmental glomerulosclerosis 9, and with ventriculomegaly with cystic kidney disease. [provided by RefSeq, Aug 2016]
Transcription factors with Perturb-seq knockdown data for CRB2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRB2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRB2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:123,349,690–123,350,320 | 5.8 kb | Proximal (<10kb) | 297 | |
| chr9:123,353,960–123,354,559 | 1.6 kb | Proximal (<10kb) | 362 | |
| chr9:123,355,973–123,356,253 | at TSS | At TSS | 102 | |
| chr9:123,359,538–123,361,097 | 3.4 kb | Proximal (<10kb) | 570 | |
| chr9:123,365,385–123,365,572 | 9.2 kb | Proximal (<10kb) | 45 | |
| chr9:123,366,156–123,366,462 | 10.0 kb | Proximal (<10kb) | 78 |
Genomic view of the CRB2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.