Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
Transcription factors with Perturb-seq knockdown data for COMT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = COMT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of COMT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:19,723,413–19,724,584 | 217.6 kb | Distal (>10kb) Multiome | 572 | |
| chr22:19,765,840–19,766,429 | 175.6 kb | Distal (>10kb) Multiome | 174 | |
| chr22:19,854,381–19,855,705 | 86.8 kb | Distal (>10kb) Multiome | 1014 | |
| chr22:19,947,834–19,948,023 | 6.0 kb | Proximal (<10kb) | 224 | |
| chr22:19,959,058–19,959,828 | 17.7 kb | Distal (>10kb) Multiome | 576 | |
| chr22:19,962,339–19,962,798 | 20.7 kb | Distal (>10kb) Multiome | 442 | |
| chr22:19,986,350–19,987,426 | 45.4 kb | Distal (>10kb) Multiome | 539 | |
| chr22:20,016,068–20,017,437 | 75.0 kb | Distal (>10kb) Multiome | 640 | |
| chr22:20,020,662–20,021,745 | 79.2 kb | Distal (>10kb) Multiome | 718 | |
| chr22:20,079,663–20,080,743 | 138.3 kb | Distal (>10kb) Multiome | 994 | |
| chr22:20,116,628–20,118,048 | 175.5 kb | Distal (>10kb) Multiome | 999 | |
| chr22:20,130,806–20,131,896 | 189.4 kb | Distal (>10kb) Multiome | 525 |
Genomic view of the COMT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.