Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms. [provided by RefSeq, Apr 2014]
Transcription factors with Perturb-seq knockdown data for COASY. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = COASY upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of COASY, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:42,275,506–42,277,052 | 285.8 kb | Distal (>10kb) Multiome | 892 | |
| chr17:42,288,257–42,288,808 | 273.6 kb | Distal (>10kb) Multiome | 449 | |
| chr17:42,312,237–42,312,887 | 249.7 kb | Distal (>10kb) Multiome | 774 | |
| chr17:42,387,693–42,389,289 | 173.5 kb | Distal (>10kb) Multiome | 1178 | |
| chr17:42,389,749–42,390,415 | 172.0 kb | Distal (>10kb) Multiome | 95 | |
| chr17:42,422,618–42,423,513 | 139.0 kb | Distal (>10kb) Multiome | 765 | |
| chr17:42,531,503–42,532,256 | 30.2 kb | Distal (>10kb) Multiome | 571 | |
| chr17:42,535,863–42,536,855 | 25.8 kb | Distal (>10kb) Multiome | 987 | |
| chr17:42,547,934–42,548,909 | 13.7 kb | Distal (>10kb) Multiome | 325 | |
| chr17:42,554,285–42,555,164 | 7.4 kb | Proximal (<10kb) Multiome | 899 | |
| chr17:42,556,290–42,556,493 | 5.7 kb | Proximal (<10kb) | 27 | |
| chr17:42,561,770–42,562,376 | 67 bp | At TSS Multiome | 905 | |
| chr17:42,566,552–42,567,732 | 4.9 kb | Proximal (<10kb) Multiome | 902 | |
| chr17:42,577,421–42,578,281 | 15.6 kb | Distal (>10kb) Multiome | 884 | |
| chr17:42,578,382–42,578,892 | 16.5 kb | Distal (>10kb) Multiome | 477 | |
| chr17:42,608,764–42,610,335 | 47.4 kb | Distal (>10kb) Multiome | 909 | |
| chr17:42,658,938–42,659,852 | 97.2 kb | Distal (>10kb) Multiome | 734 | |
| chr17:42,669,472–42,671,112 | 108.6 kb | Distal (>10kb) Multiome | 230 | |
| chr17:42,673,584–42,674,462 | 111.9 kb | Distal (>10kb) Multiome | 296 | |
| chr17:42,674,847–42,675,579 | 113.1 kb | Distal (>10kb) Multiome | 194 | |
| chr17:42,676,055–42,678,790 | 115.0 kb | Distal (>10kb) Multiome | 1070 | |
| chr17:42,679,005–42,680,519 | 117.8 kb | Distal (>10kb) Multiome | 654 | |
| chr17:42,682,736–42,683,400 | 120.6 kb | Distal (>10kb) Multiome | 480 | |
| chr17:42,744,352–42,745,375 | 182.8 kb | Distal (>10kb) Multiome | 966 | |
| chr17:42,761,003–42,761,468 | 198.9 kb | Distal (>10kb) Multiome | 335 | |
| chr17:42,780,029–42,780,899 | 218.4 kb | Distal (>10kb) Multiome | 712 | |
| chr17:42,798,408–42,798,877 | 236.6 kb | Distal (>10kb) Multiome | 866 | |
| chr17:42,823,968–42,824,503 | 262.2 kb | Distal (>10kb) Multiome | 829 | |
| chr17:42,832,889–42,833,841 | 271.2 kb | Distal (>10kb) Multiome | 980 |
Genomic view of the COASY locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.