This gene belongs to the ATP-ases associated with diverse cellular activities (AAA+) superfamily. Members of this superfamily form ring-shaped homo-hexamers and have highly conserved ATPase domains that are involved in various processes including DNA replication, protein degradation and reactivation of misfolded proteins. All members of this family hydrolyze ATP through their AAA+ domains and use the energy generated through ATP hydrolysis to exert mechanical force on their substrates. In addition to an AAA+ domain, the protein encoded by this gene contains a C-terminal D2 domain, which is characteristic of the AAA+ subfamily of Caseinolytic peptidases to which this protein belongs. It cooperates with Hsp70 in the disaggregation of protein aggregates. Allelic variants of this gene are associated with 3-methylglutaconic aciduria, which causes cataracts and neutropenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Transcription factors with Perturb-seq knockdown data for CLPB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CLPB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CLPB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:72,222,505–72,225,138 | 211.1 kb | Distal (>10kb) Multiome | 815 | |
| chr11:72,237,525–72,239,038 | 196.3 kb | Distal (>10kb) Multiome | 427 | |
| chr11:72,239,350–72,240,441 | 194.7 kb | Distal (>10kb) Multiome | 349 | |
| chr11:72,243,706–72,244,831 | 190.3 kb | Distal (>10kb) Multiome | 234 | |
| chr11:72,433,982–72,434,972 | 13 bp | At TSS Multiome | 950 | |
| chr11:72,534,428–72,535,106 | 100.4 kb | Distal (>10kb) Multiome | 152 | |
| chr11:72,584,069–72,584,889 | 149.9 kb | Distal (>10kb) Multiome | 98 | |
| chr11:72,589,653–72,590,682 | 155.7 kb | Distal (>10kb) Multiome | 303 | |
| chr11:72,642,014–72,643,645 | 208.6 kb | Distal (>10kb) Multiome | 594 | |
| chr11:72,683,647–72,685,043 | 249.7 kb | Distal (>10kb) Multiome | 428 | |
| chr11:72,722,329–72,722,710 | 288.1 kb | Distal (>10kb) Multiome | 323 |
Genomic view of the CLPB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.