The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]
Transcription factors with Perturb-seq knockdown data for CHST8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHST8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHST8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:33,373,183–33,374,325 | 248.3 kb | Distal (>10kb) Multiome | 871 | |
| chr19:33,406,730–33,407,310 | 215.0 kb | Distal (>10kb) Multiome | 393 | |
| chr19:33,520,926–33,522,397 | 100.1 kb | Distal (>10kb) Multiome | 747 | |
| chr19:33,621,458–33,623,467 | 12 bp | At TSS Multiome | 384 | |
| chr19:33,777,882–33,778,531 | 156.2 kb | Distal (>10kb) Multiome | 668 | |
| chr19:33,795,218–33,798,802 | 173.7 kb | Distal (>10kb) Multiome | 887 | |
| chr19:33,819,821–33,820,728 | 198.3 kb | Distal (>10kb) Multiome | 512 | |
| chr19:33,905,451–33,907,250 | 284.4 kb | Distal (>10kb) Multiome | 371 |
Genomic view of the CHST8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.