CHRNA5
cholinergic receptor nicotinic alpha 5 subunit

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]

Member of: DE-6 Developmental clusters: GC4
Biological processes 50 terms
acetylcholine receptor activity (GO:0015464)acetylcholine receptor signaling pathway (GO:0095500)acetylcholine receptor signaling pathway (GO:0095500)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated channel complex (GO:0005892)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)acetylcholine-gated monoatomic cation-selective channel activity (GO:0022848)behavioral response to nicotine (GO:0035095)calcium ion transport (GO:0006816)cell communication (GO:0007154)chemical synaptic transmission (GO:0007268)chemical synaptic transmission, postsynaptic (GO:0099565)cholinergic synapse (GO:0098981)dopaminergic synapse (GO:0098691)excitatory postsynaptic potential (GO:0060079)extracellular ligand-gated monoatomic ion channel activity (GO:0005230)ligand-gated monoatomic ion channel activity (GO:0015276)membrane (GO:0016020)membrane depolarization (GO:0051899)membrane depolarization (GO:0051899)monoatomic ion channel activity (GO:0005216)monoatomic ion transmembrane transport (GO:0034220)monoatomic ion transmembrane transport (GO:0034220)monoatomic ion transport (GO:0006811)neuromuscular synaptic transmission (GO:0007274)neuron projection (GO:0043005)neurotransmitter receptor activity (GO:0030594)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)plasma membrane (GO:0005886)postsynaptic membrane (GO:0045211)presynaptic membrane (GO:0042734)presynaptic modulation of chemical synaptic transmission (GO:0099171)presynaptic modulation of chemical synaptic transmission (GO:0099171)protein binding (GO:0005515)regulation of postsynaptic membrane potential (GO:0060078)response to nicotine (GO:0035094)response to nicotine (GO:0035094)signal transduction (GO:0007165)signaling (GO:0023052)synapse (GO:0045202)synaptic membrane (GO:0097060)synaptic transmission, cholinergic (GO:0007271)transmembrane signaling receptor activity (GO:0004888)
Expression (TPM)
CHRNA5 — as a Regulated Gene

TFs regulating CHRNA5 0 TFs

Transcription factors with Perturb-seq knockdown data for CHRNA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHRNA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to CHRNA5

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHRNA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr15:78,299,130–78,299,874 265.9 kb Distal (>10kb) Multiome 798
chr15:78,303,744–78,304,214 261.7 kb Distal (>10kb) Multiome 287
chr15:78,340,100–78,340,745 225.3 kb Distal (>10kb) Multiome 194
chr15:78,348,106–78,348,824 217.1 kb Distal (>10kb) Multiome 178
chr15:78,437,554–78,438,897 127.4 kb Distal (>10kb) Multiome 918
chr15:78,506,965–78,508,087 58.1 kb Distal (>10kb) Multiome 674
chr15:78,539,941–78,541,020 25.1 kb Distal (>10kb) Multiome 773
chr15:78,564,790–78,566,388 73 bp At TSS Multiome 618
chr15:78,619,950–78,621,461 54.6 kb Distal (>10kb) Multiome 274
chr15:78,640,579–78,641,869 75.8 kb Distal (>10kb) Multiome 406
chr15:78,671,569–78,672,301 106.3 kb Distal (>10kb) Multiome 254
chr15:78,810,245–78,812,147 246.0 kb Distal (>10kb) Multiome 505

Genome Browser

Genomic view of the CHRNA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr15:78,289,130 – 78,822,147
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq