This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]
Transcription factors with Perturb-seq knockdown data for CHFR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHFR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHFR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:132,686,665–132,688,078 | 268.8 kb | Distal (>10kb) Multiome | 824 | |
| chr12:132,710,174–132,711,444 | 245.6 kb | Distal (>10kb) Multiome | 940 | |
| chr12:132,761,549–132,762,490 | 194.2 kb | Distal (>10kb) Multiome | 760 | |
| chr12:132,828,453–132,829,499 | 127.2 kb | Distal (>10kb) Multiome | 930 | |
| chr12:132,887,106–132,888,371 | 68.6 kb | Distal (>10kb) Multiome | 840 | |
| chr12:132,907,962–132,909,233 | 47.7 kb | Distal (>10kb) Multiome | 481 | |
| chr12:132,955,400–132,956,621 | 34 bp | At TSS Multiome | 715 | |
| chr12:132,986,063–132,986,840 | 30.0 kb | Distal (>10kb) Multiome | 840 | |
| chr12:133,036,951–133,037,971 | 81.1 kb | Distal (>10kb) Multiome | 973 | |
| chr12:133,079,789–133,081,303 | 124.1 kb | Distal (>10kb) Multiome | 937 | |
| chr12:133,129,825–133,131,032 | 174.1 kb | Distal (>10kb) Multiome | 945 | |
| chr12:133,181,282–133,181,959 | 225.2 kb | Distal (>10kb) Multiome | 767 |
Genomic view of the CHFR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.