This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]
Transcription factors with Perturb-seq knockdown data for CHD8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHD8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHD8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:21,191,911–21,192,845 | 264.0 kb | Distal (>10kb) Multiome | 463 | |
| chr14:21,268,822–21,270,154 | 186.4 kb | Distal (>10kb) Multiome | 1029 | |
| chr14:21,308,204–21,309,328 | 147.2 kb | Distal (>10kb) Multiome | 647 | |
| chr14:21,383,896–21,384,850 | 71.8 kb | Distal (>10kb) Multiome | 931 | |
| chr14:21,436,834–21,437,890 | 18.7 kb | Distal (>10kb) Multiome | 875 | |
| chr14:21,455,683–21,456,683 | 151 bp | At TSS Multiome | 862 | |
| chr14:21,476,573–21,477,687 | 20.9 kb | Distal (>10kb) Multiome | 931 | |
| chr14:21,510,804–21,511,761 | 55.3 kb | Distal (>10kb) Multiome | 972 | |
| chr14:21,525,630–21,527,355 | 70.4 kb | Distal (>10kb) Multiome | 627 | |
| chr14:21,534,439–21,535,312 | 78.7 kb | Distal (>10kb) Multiome | 146 | |
| chr14:21,536,256–21,537,359 | 80.8 kb | Distal (>10kb) Multiome | 181 | |
| chr14:21,557,417–21,557,905 | 101.5 kb | Distal (>10kb) Multiome | 526 |
Genomic view of the CHD8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.