This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CENPB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:3,672,530–3,673,055 | 114.1 kb | Distal (>10kb) Multiome | 158 | |
| chr20:3,681,778–3,682,557 | 104.6 kb | Distal (>10kb) Multiome | 170 | |
| chr20:3,732,150–3,733,030 | 54.2 kb | Distal (>10kb) Multiome | 192 | |
| chr20:3,744,813–3,745,795 | 41.5 kb | Distal (>10kb) Multiome | 379 | |
| chr20:3,749,619–3,750,251 | 36.8 kb | Distal (>10kb) Multiome | 520 | |
| chr20:3,751,352–3,752,738 | 34.6 kb | Distal (>10kb) Multiome | 261 | |
| chr20:3,767,155–3,768,879 | 18.8 kb | Distal (>10kb) Multiome | 1011 | |
| chr20:3,777,516–3,778,624 | 8.7 kb | Proximal (<10kb) Multiome | 428 | |
| chr20:3,781,161–3,781,797 | 5.3 kb | Proximal (<10kb) Multiome | 595 | |
| chr20:3,785,713–3,787,025 | 65 bp | At TSS Multiome | 695 | |
| chr20:3,795,543–3,796,972 | 9.4 kb | Proximal (<10kb) Multiome | 925 | |
| chr20:3,799,527–3,800,087 | 13.0 kb | Distal (>10kb) Multiome | 197 | |
| chr20:3,820,073–3,821,115 | 33.8 kb | Distal (>10kb) Multiome | 1081 | |
| chr20:3,846,214–3,847,496 | 60.1 kb | Distal (>10kb) Multiome | 940 | |
| chr20:3,888,329–3,890,033 | 102.4 kb | Distal (>10kb) Multiome | 1031 | |
| chr20:4,014,730–4,016,019 | 228.9 kb | Distal (>10kb) Multiome | 865 | |
| chr20:4,064,484–4,064,955 | 277.9 kb | Distal (>10kb) Multiome | 290 | |
| chr20:4,082,773–4,083,490 | 296.4 kb | Distal (>10kb) Multiome HiCAR | 445 | |
| chr20:4,085,118–4,085,823 | 298.7 kb | Distal (>10kb) Multiome HiCAR | 674 |
Genomic view of the CENPB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.