CDK5R1
cyclin dependent kinase 5 regulatory subunit 1 | Nck5a, p35, p35nck5a

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]

Developmental clusters: GC2
Biological processes 85 terms
G protein-coupled acetylcholine receptor signaling pathway (GO:0007213)actin binding (GO:0003779)actin filament binding (GO:0051015)alpha-tubulin binding (GO:0043014)alpha-tubulin binding (GO:0043014)axon (GO:0030424)axon guidance (GO:0007411)axon guidance (GO:0007411)axon guidance (GO:0007411)axonal fasciculation (GO:0007413)axonal fasciculation (GO:0007413)beta-tubulin binding (GO:0048487)beta-tubulin binding (GO:0048487)brain development (GO:0007420)brain development (GO:0007420)brain development (GO:0007420)brain development (GO:0007420)cadherin binding (GO:0045296)calcium ion binding (GO:0005509)cerebral cortex radially oriented cell migration (GO:0021799)contractile muscle fiber (GO:0043292)cyclin-dependent protein serine/threonine kinase activator activity (GO:0061575)cyclin-dependent protein serine/threonine kinase activator activity (GO:0061575)cyclin-dependent protein serine/threonine kinase activator activity (GO:0061575)cyclin-dependent protein serine/threonine kinase activator activity (GO:0061575)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)dendrite (GO:0030425)dendritic spine (GO:0043197)ephrin receptor signaling pathway (GO:0048013)ephrin receptor signaling pathway (GO:0048013)growth cone (GO:0030426)growth cone (GO:0030426)ionotropic glutamate receptor binding (GO:0035255)ionotropic glutamate receptor signaling pathway (GO:0035235)kinase activity (GO:0016301)membrane (GO:0016020)microtubule cytoskeleton organization (GO:0000226)negative regulation of DNA-templated transcription (GO:0045892)neuromuscular junction (GO:0031594)neuron cell-cell adhesion (GO:0007158)neuron cell-cell adhesion (GO:0007158)neuron differentiation (GO:0030182)neuron migration (GO:0001764)neuron migration (GO:0001764)neuron projection (GO:0043005)neuron projection (GO:0043005)neuron projection development (GO:0031175)neuron projection development (GO:0031175)neuronal cell body (GO:0043025)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)peptidyl-serine phosphorylation (GO:0018105)peptidyl-threonine phosphorylation (GO:0018107)perikaryon (GO:0043204)perinuclear region of cytoplasm (GO:0048471)perinuclear region of cytoplasm (GO:0048471)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of MHC class II biosynthetic process (GO:0045348)positive regulation of microtubule polymerization (GO:0031116)positive regulation of neuron apoptotic process (GO:0043525)positive regulation of tumor necrosis factor-mediated signaling pathway (GO:1903265)postsynaptic density (GO:0014069)protease binding (GO:0002020)protein binding (GO:0005515)protein kinase 5 complex (GO:0016533)protein kinase activator activity (GO:0030295)protein kinase activity (GO:0004672)protein kinase binding (GO:0019901)protein kinase binding (GO:0019901)protein kinase binding (GO:0019901)protein serine/threonine kinase activator activity (GO:0043539)protein serine/threonine kinase activator activity (GO:0043539)regulation of actin cytoskeleton organization (GO:0032956)regulation of cyclin-dependent protein serine/threonine kinase activity (GO:0000079)regulation of dendritic spine morphogenesis (GO:0061001)regulation of dendritic spine morphogenesis (GO:0061001)regulation of macroautophagy (GO:0016241)regulation of microtubule cytoskeleton organization (GO:0070507)regulation of neuron differentiation (GO:0045664)
Expression (TPM)
CDK5R1 — as a Regulated Gene

TFs regulating CDK5R1 0 TFs

Transcription factors with Perturb-seq knockdown data for CDK5R1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDK5R1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to CDK5R1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDK5R1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr17:32,483,367–32,483,985 3.0 kb Proximal (<10kb) 306
chr17:32,486,205–32,487,756 at TSS At TSS 682

Genome Browser

Genomic view of the CDK5R1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr17:32,473,367 – 32,497,756
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq