The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Transcription factors with Perturb-seq knockdown data for CDC45. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDC45 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDC45, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:19,290,875–19,292,389 | 188.1 kb | Distal (>10kb) Multiome | 854 | |
| chr22:19,431,488–19,432,799 | 47.5 kb | Distal (>10kb) Multiome | 1140 | |
| chr22:19,446,962–19,448,379 | 32.2 kb | Distal (>10kb) Multiome | 889 | |
| chr22:19,478,898–19,480,311 | 244 bp | At TSS Multiome | 867 | |
| chr22:19,523,289–19,524,793 | 43.7 kb | Distal (>10kb) Multiome | 242 | |
| chr22:19,717,840–19,718,731 | 238.3 kb | Distal (>10kb) Multiome | 592 | |
| chr22:19,723,413–19,724,584 | 244.3 kb | Distal (>10kb) Multiome | 572 | |
| chr22:19,730,516–19,731,296 | 251.0 kb | Distal (>10kb) Multiome | 743 | |
| chr22:19,765,840–19,766,429 | 286.2 kb | Distal (>10kb) Multiome | 174 |
Genomic view of the CDC45 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.