CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CDC25B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CDC25B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CDC25B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:3,681,778–3,682,557 | 114.1 kb | Distal (>10kb) Multiome | 170 | |
| chr20:3,732,150–3,733,030 | 63.7 kb | Distal (>10kb) Multiome | 192 | |
| chr20:3,744,813–3,745,795 | 51.1 kb | Distal (>10kb) Multiome | 379 | |
| chr20:3,749,619–3,750,251 | 46.4 kb | Distal (>10kb) Multiome | 520 | |
| chr20:3,751,352–3,752,738 | 44.1 kb | Distal (>10kb) Multiome | 261 | |
| chr20:3,767,155–3,768,879 | 28.4 kb | Distal (>10kb) Multiome | 1011 | |
| chr20:3,777,516–3,778,624 | 18.2 kb | Distal (>10kb) Multiome | 428 | |
| chr20:3,781,161–3,781,797 | 14.8 kb | Distal (>10kb) Multiome | 595 | |
| chr20:3,785,713–3,787,025 | 9.5 kb | Proximal (<10kb) Multiome | 695 | |
| chr20:3,795,543–3,796,972 | 173 bp | At TSS Multiome | 925 | |
| chr20:3,799,527–3,800,087 | 3.8 kb | Proximal (<10kb) | 197 | |
| chr20:3,820,073–3,821,115 | 24.2 kb | Distal (>10kb) Multiome | 1081 | |
| chr20:3,846,214–3,847,496 | 50.5 kb | Distal (>10kb) Multiome | 940 | |
| chr20:3,875,544–3,876,019 | 79.6 kb | Distal (>10kb) Multiome | 80 | |
| chr20:3,888,329–3,890,033 | 92.9 kb | Distal (>10kb) Multiome | 1031 | |
| chr20:4,014,730–4,016,019 | 219.3 kb | Distal (>10kb) Multiome | 865 | |
| chr20:4,085,118–4,085,823 | 289.2 kb | Distal (>10kb) Multiome | 674 |
Genomic view of the CDC25B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.