This gene encodes a sperm tail protein that is highly expressed in adult testis, spermatocytes and spermatids. The protein plays a critical role in the assembly of the nexin-dynein regulatory complex. Mutations in this gene result in primary ciliary dyskinesia. [provided by RefSeq, Nov 2013]
Transcription factors with Perturb-seq knockdown data for CCDC65. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CCDC65 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CCDC65, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:48,681,532–48,682,844 | 221.8 kb | Distal (>10kb) Multiome | 933 | |
| chr12:48,716,299–48,717,435 | 187.2 kb | Distal (>10kb) Multiome | 1077 | |
| chr12:48,788,600–48,789,901 | 114.8 kb | Distal (>10kb) Multiome | 478 | |
| chr12:48,795,703–48,796,330 | 108.1 kb | Distal (>10kb) Multiome | 333 | |
| chr12:48,814,087–48,815,981 | 89.3 kb | Distal (>10kb) Multiome | 815 | |
| chr12:48,817,927–48,819,273 | 85.6 kb | Distal (>10kb) Multiome | 459 | |
| chr12:48,851,862–48,852,825 | 51.9 kb | Distal (>10kb) Multiome HiCAR | 900 | |
| chr12:48,864,974–48,866,292 | 38.2 kb | Distal (>10kb) Multiome | 649 | |
| chr12:48,883,231–48,884,168 | 20.3 kb | Distal (>10kb) Multiome | 433 | |
| chr12:48,890,671–48,891,380 | 13.2 kb | Distal (>10kb) Multiome HiCAR | 517 | |
| chr12:48,903,980–48,904,381 | at TSS | At TSS | 332 | |
| chr12:48,924,659–48,925,688 | 20.8 kb | Distal (>10kb) Multiome | 413 | |
| chr12:48,956,252–48,957,974 | 52.4 kb | Distal (>10kb) Multiome | 831 | |
| chr12:48,978,055–48,978,819 | 74.2 kb | Distal (>10kb) Multiome | 360 | |
| chr12:48,980,647–48,981,153 | 76.8 kb | Distal (>10kb) Multiome | 259 | |
| chr12:48,997,612–48,998,829 | 94.2 kb | Distal (>10kb) Multiome | 620 | |
| chr12:49,018,007–49,019,184 | 114.6 kb | Distal (>10kb) Multiome | 899 | |
| chr12:49,059,883–49,061,347 | 156.7 kb | Distal (>10kb) Multiome | 1060 | |
| chr12:49,069,381–49,070,589 | 165.9 kb | Distal (>10kb) Multiome | 804 | |
| chr12:49,088,153–49,088,744 | 184.3 kb | Distal (>10kb) Multiome | 473 | |
| chr12:49,109,990–49,111,248 | 206.7 kb | Distal (>10kb) Multiome | 693 | |
| chr12:49,129,851–49,132,062 | 226.2 kb | Distal (>10kb) Multiome | 1233 | |
| chr12:49,188,266–49,189,371 | 285.0 kb | Distal (>10kb) Multiome | 676 |
Genomic view of the CCDC65 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.