This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for CASP9. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CASP9 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CASP9, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:15,246,891–15,247,752 | 276.9 kb | Distal (>10kb) Multiome | 362 | |
| chr1:15,409,150–15,410,227 | 114.4 kb | Distal (>10kb) Multiome | 618 | |
| chr1:15,412,068–15,412,674 | 111.9 kb | Distal (>10kb) Multiome | 120 | |
| chr1:15,523,864–15,525,570 | 450 bp | At TSS Multiome | 674 | |
| chr1:15,526,118–15,527,442 | 2.6 kb | Proximal (<10kb) Multiome | 899 | |
| chr1:15,584,405–15,585,197 | 60.6 kb | Distal (>10kb) Multiome | 693 | |
| chr1:15,603,163–15,603,936 | 79.4 kb | Distal (>10kb) Multiome | 795 | |
| chr1:15,616,785–15,618,260 | 93.1 kb | Distal (>10kb) Multiome | 909 | |
| chr1:15,676,618–15,677,408 | 152.9 kb | Distal (>10kb) Multiome | 95 | |
| chr1:15,741,258–15,741,859 | 217.3 kb | Distal (>10kb) Multiome | 436 | |
| chr1:15,757,615–15,759,609 | 234.4 kb | Distal (>10kb) Multiome | 439 | |
| chr1:15,799,931–15,800,776 | 276.1 kb | Distal (>10kb) Multiome | 734 |
Genomic view of the CASP9 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.