Transcription factors with Perturb-seq knockdown data for CASC2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CASC2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CASC2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:117,830,493–117,831,491 | 216.0 kb | Distal (>10kb) Multiome | 253 | |
| chr10:118,045,936–118,047,525 | 127 bp | At TSS Multiome | 849 | |
| chr10:118,258,175–118,258,829 | 211.5 kb | Distal (>10kb) Multiome | 139 | |
| chr10:118,341,772–118,342,929 | 295.3 kb | Distal (>10kb) Multiome | 675 |
Genomic view of the CASC2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.