This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]
Transcription factors with Perturb-seq knockdown data for CARS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CARS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CARS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:2,862,328–2,863,500 | 194.8 kb | Distal (>10kb) Multiome | 132 | |
| chr11:2,883,795–2,886,948 | 171.5 kb | Distal (>10kb) Multiome | 639 | |
| chr11:2,891,828–2,893,111 | 164.9 kb | Distal (>10kb) Multiome | 572 | |
| chr11:2,901,621–2,903,073 | 155.2 kb | Distal (>10kb) Multiome | 610 | |
| chr11:2,928,807–2,931,249 | 126.5 kb | Distal (>10kb) Multiome | 770 | |
| chr11:2,991,519–2,993,201 | 65.1 kb | Distal (>10kb) Multiome | 759 | |
| chr11:3,056,953–3,057,972 | 126 bp | At TSS Multiome | 798 | |
| chr11:3,063,983–3,064,142 | 6.6 kb | Proximal (<10kb) | 34 | |
| chr11:3,091,645–3,092,317 | 34.5 kb | Distal (>10kb) Multiome | 449 | |
| chr11:3,148,512–3,149,454 | 91.6 kb | Distal (>10kb) Multiome | 152 | |
| chr11:3,160,043–3,161,026 | 103.1 kb | Distal (>10kb) Multiome | 283 | |
| chr11:3,164,925–3,165,485 | 107.8 kb | Distal (>10kb) Multiome | 326 |
Genomic view of the CARS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.