The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for CARD10. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CARD10 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CARD10, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:37,419,824–37,420,580 | 99.1 kb | Distal (>10kb) Multiome | 150 | |
| chr22:37,518,831–37,519,884 | 8 bp | At TSS Multiome | 548 | |
| chr22:37,545,569–37,546,885 | 26.9 kb | Distal (>10kb) Multiome | 682 | |
| chr22:37,559,893–37,560,965 | 40.9 kb | Distal (>10kb) Multiome | 589 | |
| chr22:37,608,044–37,609,455 | 89.4 kb | Distal (>10kb) Multiome | 920 | |
| chr22:37,639,157–37,639,951 | 120.1 kb | Distal (>10kb) Multiome | 427 | |
| chr22:37,658,172–37,659,508 | 139.2 kb | Distal (>10kb) Multiome | 722 | |
| chr22:37,674,916–37,675,641 | 156.0 kb | Distal (>10kb) Multiome | 607 | |
| chr22:37,676,590–37,677,674 | 157.8 kb | Distal (>10kb) Multiome | 655 | |
| chr22:37,686,104–37,686,784 | 167.0 kb | Distal (>10kb) Multiome | 866 | |
| chr22:37,696,496–37,697,685 | 177.5 kb | Distal (>10kb) Multiome | 428 | |
| chr22:37,745,641–37,746,995 | 226.7 kb | Distal (>10kb) Multiome | 627 | |
| chr22:37,802,124–37,802,989 | 283.1 kb | Distal (>10kb) Multiome | 603 | |
| chr22:37,804,679–37,805,768 | 285.8 kb | Distal (>10kb) Multiome | 763 | |
| chr22:37,807,436–37,808,267 | 288.4 kb | Distal (>10kb) Multiome | 766 | |
| chr22:37,817,672–37,819,114 | 299.7 kb | Distal (>10kb) Multiome | 713 |
Genomic view of the CARD10 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.