Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes carbonic anhydrase 5B. CA5B, and the related CA5A gene, has its expression localized in the mitochondria though CA5B has a wider tissue distribution than CA5A, which is restricted to the liver, kidneys, and skeletal muscle. A carbonic anhydrase pseudogene (CA5BP1) is adjacent to the CA5B gene and these two loci produce CA5BP1-CA5B readthrough transcripts. [provided by RefSeq, Jan 2019]
Transcription factors with Perturb-seq knockdown data for CA5B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CA5B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CA5B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:15,493,050–15,493,972 | 244.9 kb | Distal (>10kb) Multiome | 563 | |
| chrX:15,674,577–15,676,009 | 62.9 kb | Distal (>10kb) Multiome | 724 | |
| chrX:15,737,668–15,738,738 | 147 bp | At TSS Multiome | 369 | |
| chrX:15,747,284–15,747,543 | 9.0 kb | Proximal (<10kb) | 18 | |
| chrX:15,747,676–15,748,135 | 9.4 kb | Proximal (<10kb) | 41 | |
| chrX:15,789,952–15,790,771 | 52.2 kb | Distal (>10kb) Multiome | 531 | |
| chrX:15,854,233–15,855,558 | 116.7 kb | Distal (>10kb) Multiome | 706 | |
| chrX:15,874,284–15,875,274 | 136.5 kb | Distal (>10kb) Multiome | 139 |
Genomic view of the CA5B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.