Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]
Transcription factors with Perturb-seq knockdown data for CA12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CA12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CA12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:63,121,504–63,122,682 | 260.0 kb | Distal (>10kb) Multiome | 750 | |
| chr15:63,157,102–63,158,166 | 224.3 kb | Distal (>10kb) Multiome | 954 | |
| chr15:63,188,797–63,190,016 | 192.4 kb | Distal (>10kb) Multiome | 573 | |
| chr15:63,277,063–63,278,175 | 104.4 kb | Distal (>10kb) Multiome | 651 | |
| chr15:63,381,566–63,382,221 | 22 bp | At TSS Multiome | 358 | |
| chr15:63,396,367–63,396,999 | 14.9 kb | Distal (>10kb) Multiome | 284 | |
| chr15:63,434,760–63,435,736 | 53.2 kb | Distal (>10kb) Multiome | 102 | |
| chr15:63,466,399–63,467,254 | 85.0 kb | Distal (>10kb) Multiome | 497 | |
| chr15:63,502,962–63,505,773 | 121.4 kb | Distal (>10kb) Multiome | 1114 | |
| chr15:63,599,386–63,601,708 | 219.1 kb | Distal (>10kb) Multiome | 832 |
Genomic view of the CA12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.