Transcription factors with Perturb-seq knockdown data for C5orf22. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C5orf22 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C5orf22, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:31,426,930–31,427,798 | 104.9 kb | Distal (>10kb) Multiome | 133 | |
| chr5:31,531,635–31,532,846 | 64 bp | At TSS Multiome | 818 | |
| chr5:31,618,104–31,619,083 | 86.5 kb | Distal (>10kb) Multiome | 172 | |
| chr5:31,638,486–31,640,394 | 106.8 kb | Distal (>10kb) Multiome | 551 | |
| chr5:31,815,109–31,815,703 | 283.0 kb | Distal (>10kb) Multiome | 23 |
Genomic view of the C5orf22 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.