Transcription factors with Perturb-seq knockdown data for C12orf56. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C12orf56 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C12orf56, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:63,779,430–63,780,603 | 610.8 kb | Distal (>10kb) Multiome HiCAR | 817 | |
| chr12:63,843,303–63,845,210 | 546.9 kb | Distal (>10kb) Multiome HiCAR | 940 | |
| chr12:64,221,528–64,222,890 | 168.5 kb | Distal (>10kb) Multiome | 970 | |
| chr12:64,390,059–64,390,977 | 157 bp | At TSS Multiome | 268 | |
| chr12:64,392,336–64,392,511 | 1.6 kb | Proximal (<10kb) | 27 | |
| chr12:64,400,113–64,400,472 | 9.4 kb | Proximal (<10kb) | 88 | |
| chr12:64,403,794–64,405,423 | 13.6 kb | Distal (>10kb) Multiome | 1007 | |
| chr12:64,451,663–64,452,624 | 61.4 kb | Distal (>10kb) Multiome | 977 | |
| chr12:64,543,521–64,544,182 | 153.0 kb | Distal (>10kb) Multiome | 54 | |
| chr12:64,608,914–64,611,341 | 219.6 kb | Distal (>10kb) Multiome | 922 |
Genomic view of the C12orf56 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.