Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
Transcription factors with Perturb-seq knockdown data for BTN2A2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BTN2A2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BTN2A2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:26,087,383–26,088,017 | 295.4 kb | Distal (>10kb) Multiome | 572 | |
| chr6:26,103,452–26,105,109 | 279.2 kb | Distal (>10kb) Multiome | 1169 | |
| chr6:26,122,481–26,124,909 | 260.2 kb | Distal (>10kb) Multiome | 1216 | |
| chr6:26,156,184–26,158,744 | 225.1 kb | Distal (>10kb) Multiome | 1310 | |
| chr6:26,171,798–26,172,499 | 211.1 kb | Distal (>10kb) Multiome | 580 | |
| chr6:26,183,128–26,184,377 | 199.5 kb | Distal (>10kb) Multiome | 311 | |
| chr6:26,187,874–26,189,538 | 194.0 kb | Distal (>10kb) Multiome | 1053 | |
| chr6:26,196,695–26,197,676 | 185.9 kb | Distal (>10kb) Multiome | 842 | |
| chr6:26,198,203–26,200,197 | 183.7 kb | Distal (>10kb) Multiome | 939 | |
| chr6:26,203,791–26,205,119 | 178.6 kb | Distal (>10kb) Multiome | 1071 | |
| chr6:26,214,872–26,218,468 | 166.3 kb | Distal (>10kb) Multiome | 1096 | |
| chr6:26,223,716–26,224,480 | 159.1 kb | Distal (>10kb) Multiome | 507 | |
| chr6:26,224,856–26,226,566 | 157.9 kb | Distal (>10kb) Multiome | 451 | |
| chr6:26,239,944–26,240,920 | 142.8 kb | Distal (>10kb) Multiome | 375 | |
| chr6:26,249,682–26,251,138 | 132.8 kb | Distal (>10kb) Multiome | 731 | |
| chr6:26,251,339–26,252,079 | 131.4 kb | Distal (>10kb) Multiome | 445 | |
| chr6:26,270,815–26,273,967 | 110.2 kb | Distal (>10kb) Multiome | 742 | |
| chr6:26,285,011–26,286,121 | 97.5 kb | Distal (>10kb) Multiome | 1121 | |
| chr6:26,304,972–26,305,585 | 77.7 kb | Distal (>10kb) Multiome | 586 | |
| chr6:26,312,505–26,313,475 | 70.1 kb | Distal (>10kb) Multiome | 896 | |
| chr6:26,322,092–26,323,232 | 60.5 kb | Distal (>10kb) Multiome | 811 | |
| chr6:26,327,476–26,328,318 | 55.0 kb | Distal (>10kb) Multiome | 716 | |
| chr6:26,341,545–26,342,139 | 41.3 kb | Distal (>10kb) Multiome | 716 | |
| chr6:26,365,073–26,365,647 | 17.8 kb | Distal (>10kb) Multiome | 587 | |
| chr6:26,382,957–26,383,951 | 85 bp | At TSS Multiome | 448 | |
| chr6:26,421,258–26,421,866 | 38.3 kb | Distal (>10kb) Multiome | 473 | |
| chr6:26,457,390–26,458,395 | 74.7 kb | Distal (>10kb) Multiome | 525 | |
| chr6:26,473,842–26,474,357 | 91.0 kb | Distal (>10kb) Multiome | 487 | |
| chr6:26,520,324–26,522,398 | 138.9 kb | Distal (>10kb) Multiome | 1082 | |
| chr6:26,532,765–26,533,409 | 150.0 kb | Distal (>10kb) Multiome | 830 | |
| chr6:26,537,180–26,538,912 | 154.4 kb | Distal (>10kb) Multiome | 1114 | |
| chr6:26,550,296–26,550,971 | 167.6 kb | Distal (>10kb) Multiome | 695 | |
| chr6:26,553,165–26,553,642 | 170.5 kb | Distal (>10kb) Multiome | 561 | |
| chr6:26,556,432–26,557,038 | 173.4 kb | Distal (>10kb) Multiome | 676 | |
| chr6:26,571,613–26,572,233 | 188.8 kb | Distal (>10kb) Multiome | 861 | |
| chr6:26,595,619–26,596,282 | 212.8 kb | Distal (>10kb) Multiome | 798 | |
| chr6:26,596,497–26,597,408 | 213.9 kb | Distal (>10kb) Multiome | 940 | |
| chr6:26,659,338–26,660,351 | 276.6 kb | Distal (>10kb) Multiome | 748 |
Genomic view of the BTN2A2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.