BRF1

BRF1 general transcription factor IIIB subunit | BRF, TFIIIB90, hBRF, GTF3B, TAF3B2, TAF3C

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]

Member of: DE-2

Biological processes 19 terms

DNA-templated transcription initiation (GO:0006352)DNA-templated transcription initiation (GO:0006352)RNA polymerase III general transcription initiation factor activity (GO:0000995)RNA polymerase III type 3 promoter sequence-specific DNA binding (GO:0001006)TBP-class protein binding (GO:0017025)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)rRNA transcription (GO:0009303)tRNA transcription (GO:0009304)transcription by RNA polymerase III (GO:0006383)transcription by RNA polymerase III (GO:0006383)transcription factor TFIIIB complex (GO:0000126)transcription factor TFIIIB complex (GO:0000126)transcription initiation at RNA polymerase III promoter (GO:0006384)transcription preinitiation complex (GO:0097550)transcription preinitiation complex assembly (GO:0070897)

Expression (TPM)

TFs regulating BRF1 0 TFs

Transcription factors with Perturb-seq knockdown data for BRF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BRF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to BRF1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BRF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr14:104,967,607–104,968,024	280.6 kb	Distal (>10kb) Multiome	196
chr14:104,975,803–104,976,561	272.4 kb	Distal (>10kb) Multiome	394
chr14:104,977,531–104,979,147	270.1 kb	Distal (>10kb) Multiome	387
chr14:104,985,398–104,986,377	262.8 kb	Distal (>10kb) Multiome	518
chr14:105,020,271–105,022,072	227.3 kb	Distal (>10kb) Multiome	813
chr14:105,045,704–105,046,235	202.7 kb	Distal (>10kb) Multiome	635
chr14:105,086,627–105,087,884	161.5 kb	Distal (>10kb) Multiome	590
chr14:105,090,382–105,091,009	157.8 kb	Distal (>10kb) Multiome	375
chr14:105,092,908–105,094,307	154.6 kb	Distal (>10kb) Multiome	495
chr14:105,167,331–105,169,463	79.5 kb	Distal (>10kb) Multiome	496
chr14:105,180,714–105,181,828	67.3 kb	Distal (>10kb) Multiome	392
chr14:105,248,175–105,249,386	16 bp	At TSS Multiome	403
chr14:105,299,994–105,301,984	52.8 kb	Distal (>10kb) Multiome	829
chr14:105,313,768–105,315,755	66.1 kb	Distal (>10kb) Multiome	584
chr14:105,352,048–105,352,515	103.8 kb	Distal (>10kb) Multiome	344
chr14:105,398,137–105,398,986	150.0 kb	Distal (>10kb) Multiome	870
chr14:105,411,005–105,411,818	162.9 kb	Distal (>10kb) Multiome	384
chr14:105,414,940–105,415,397	166.6 kb	Distal (>10kb) Multiome	447
chr14:105,419,281–105,420,109	171.3 kb	Distal (>10kb) Multiome	570
chr14:105,448,471–105,448,924	200.2 kb	Distal (>10kb) Multiome	304
chr14:105,473,498–105,475,498	225.8 kb	Distal (>10kb) Multiome	581
chr14:105,480,543–105,482,375	232.5 kb	Distal (>10kb) Multiome	784
chr14:105,485,648–105,486,379	237.4 kb	Distal (>10kb) Multiome	424
chr14:105,486,576–105,487,923	238.7 kb	Distal (>10kb) Multiome	369
chr14:105,489,310–105,492,133	242.5 kb	Distal (>10kb) Multiome	1014
chr14:105,526,110–105,527,233	278.0 kb	Distal (>10kb) Multiome	270
chr14:105,528,085–105,528,965	280.0 kb	Distal (>10kb) Multiome	568

Genome Browser

Genomic view of the BRF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.