This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Transcription factors with Perturb-seq knockdown data for BRAF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BRAF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BRAF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:140,639,882–140,641,732 | 283.9 kb | Distal (>10kb) Multiome | 486 | |
| chr7:140,672,673–140,674,052 | 251.8 kb | Distal (>10kb) Multiome | 648 | |
| chr7:140,696,318–140,697,705 | 227.9 kb | Distal (>10kb) Multiome | 1002 | |
| chr7:140,924,039–140,925,334 | 146 bp | At TSS Multiome | 930 | |
| chr7:141,014,405–141,015,593 | 90.3 kb | Distal (>10kb) Multiome | 1023 | |
| chr7:141,071,165–141,071,881 | 146.8 kb | Distal (>10kb) Multiome | 334 | |
| chr7:141,072,522–141,074,991 | 148.6 kb | Distal (>10kb) Multiome | 574 |
Genomic view of the BRAF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.