Transcription factors with Perturb-seq knockdown data for BOLA2P3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BOLA2P3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BOLA2P3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:21,593,301–21,595,945 | 6.2 kb | Proximal (<10kb) | 834 | |
| chr6:21,596,125–21,596,441 | 5.7 kb | Proximal (<10kb) | 289 | |
| chr6:21,596,549–21,596,984 | 5.1 kb | Proximal (<10kb) | 373 | |
| chr6:21,601,865–21,602,374 | at TSS | At TSS | 104 | |
| chr6:21,609,657–21,610,431 | 7.5 kb | Proximal (<10kb) | 133 |
Genomic view of the BOLA2P3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.