Transcription factors with Perturb-seq knockdown data for BLACAT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BLACAT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BLACAT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:205,210,792–205,212,030 | 245.5 kb | Distal (>10kb) Multiome | 1103 | |
| chr1:205,227,416–205,228,381 | 229.2 kb | Distal (>10kb) Multiome | 495 | |
| chr1:205,229,263–205,229,745 | 227.5 kb | Distal (>10kb) Multiome | 331 | |
| chr1:205,300,603–205,302,127 | 155.5 kb | Distal (>10kb) Multiome | 660 | |
| chr1:205,313,308–205,314,407 | 143.1 kb | Distal (>10kb) Multiome | 327 | |
| chr1:205,321,242–205,322,079 | 135.3 kb | Distal (>10kb) Multiome | 705 | |
| chr1:205,343,395–205,344,420 | 113.1 kb | Distal (>10kb) Multiome | 395 | |
| chr1:205,430,507–205,431,109 | 26.2 kb | Distal (>10kb) Multiome | 387 | |
| chr1:205,440,982–205,441,976 | 15.9 kb | Distal (>10kb) Multiome HiCAR | 341 | |
| chr1:205,444,530–205,445,576 | 12.0 kb | Distal (>10kb) Multiome HiCAR | 351 | |
| chr1:205,447,468–205,447,874 | 9.6 kb | Proximal (<10kb) Multiome HiCAR | 135 | |
| chr1:205,448,352–205,449,283 | 8.4 kb | Proximal (<10kb) Multiome HiCAR | 269 | |
| chr1:205,449,573–205,450,965 | 6.9 kb | Proximal (<10kb) Multiome HiCAR | 512 | |
| chr1:205,451,988–205,452,191 | 3.9 kb | Proximal (<10kb) | 40 | |
| chr1:205,454,950–205,455,380 | 664 bp | At TSS | 72 | |
| chr1:205,455,490–205,456,122 | at TSS | At TSS | 94 | |
| chr1:205,456,289–205,457,681 | 206 bp | At TSS Multiome | 383 | |
| chr1:205,485,282–205,485,813 | 28.5 kb | Distal (>10kb) Multiome | 590 | |
| chr1:205,504,221–205,505,625 | 47.6 kb | Distal (>10kb) Multiome | 631 | |
| chr1:205,528,647–205,529,482 | 72.0 kb | Distal (>10kb) Multiome HiCAR | 390 | |
| chr1:205,543,280–205,544,028 | 86.5 kb | Distal (>10kb) Multiome | 371 | |
| chr1:205,591,729–205,592,393 | 135.0 kb | Distal (>10kb) Multiome | 624 | |
| chr1:205,631,034–205,632,587 | 174.8 kb | Distal (>10kb) Multiome | 1054 | |
| chr1:205,679,697–205,680,913 | 223.4 kb | Distal (>10kb) Multiome | 568 | |
| chr1:205,748,908–205,750,848 | 292.5 kb | Distal (>10kb) Multiome | 1074 |
Genomic view of the BLACAT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.