Transcription factors with Perturb-seq knockdown data for BISPR. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BISPR upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BISPR, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:17,397,300–17,397,562 | 8.1 kb | Proximal (<10kb) | 15 | |
| chr19:17,398,863–17,399,537 | 6.1 kb | Proximal (<10kb) | 132 | |
| chr19:17,403,087–17,404,355 | 1.3 kb | Proximal (<10kb) | 211 | |
| chr19:17,405,595–17,405,760 | at TSS | At TSS | 440 |
Genomic view of the BISPR locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.