The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]
Transcription factors with Perturb-seq knockdown data for BCAR1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BCAR1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BCAR1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:74,984,580–74,985,562 | 266.5 kb | Distal (>10kb) Multiome | 877 | |
| chr16:74,998,533–75,000,404 | 252.7 kb | Distal (>10kb) Multiome | 884 | |
| chr16:75,110,910–75,111,744 | 140.3 kb | Distal (>10kb) Multiome | 720 | |
| chr16:75,148,292–75,149,298 | 103.0 kb | Distal (>10kb) Multiome | 970 | |
| chr16:75,245,071–75,245,668 | 6.0 kb | Proximal (<10kb) | 765 | |
| chr16:75,250,387–75,252,500 | 71 bp | At TSS Multiome | 811 | |
| chr16:75,253,427–75,253,775 | 1.8 kb | Proximal (<10kb) | 129 | |
| chr16:75,265,114–75,266,526 | 14.4 kb | Distal (>10kb) Multiome | 798 | |
| chr16:75,432,825–75,434,426 | 181.9 kb | Distal (>10kb) Multiome | 1143 | |
| chr16:75,463,598–75,465,197 | 213.1 kb | Distal (>10kb) Multiome | 999 | |
| chr16:75,494,705–75,495,929 | 243.8 kb | Distal (>10kb) Multiome | 341 | |
| chr16:75,516,521–75,518,019 | 265.8 kb | Distal (>10kb) Multiome | 921 | |
| chr16:75,555,188–75,556,662 | 303.6 kb | Distal (>10kb) Multiome | 900 | |
| chr16:75,565,779–75,567,123 | 314.7 kb | Distal (>10kb) Multiome | 834 |
Genomic view of the BCAR1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.