The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Dec 2019]
Transcription factors with Perturb-seq knockdown data for BAD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BAD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BAD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:63,986,198–63,987,213 | 298.2 kb | Distal (>10kb) Multiome | 895 | |
| chr11:63,998,533–64,001,109 | 285.3 kb | Distal (>10kb) Multiome | 414 | |
| chr11:64,028,126–64,028,898 | 256.1 kb | Distal (>10kb) Multiome | 168 | |
| chr11:64,165,638–64,166,698 | 118.5 kb | Distal (>10kb) Multiome | 811 | |
| chr11:64,184,927–64,186,817 | 98.8 kb | Distal (>10kb) Multiome | 1091 | |
| chr11:64,225,548–64,226,862 | 58.5 kb | Distal (>10kb) Multiome | 776 | |
| chr11:64,230,074–64,231,130 | 54.2 kb | Distal (>10kb) Multiome | 715 | |
| chr11:64,233,950–64,235,296 | 50.2 kb | Distal (>10kb) Multiome | 590 | |
| chr11:64,240,398–64,242,687 | 43.4 kb | Distal (>10kb) Multiome | 982 | |
| chr11:64,246,274–64,248,065 | 37.6 kb | Distal (>10kb) Multiome | 802 | |
| chr11:64,250,699–64,251,842 | 33.3 kb | Distal (>10kb) Multiome | 498 | |
| chr11:64,268,509–64,270,920 | 14.6 kb | Distal (>10kb) Multiome | 1093 | |
| chr11:64,271,352–64,271,994 | 13.1 kb | Distal (>10kb) Multiome | 595 | |
| chr11:64,284,095–64,286,666 | 1.1 kb | Proximal (<10kb) Multiome | 807 | |
| chr11:64,300,129–64,301,268 | 16.3 kb | Distal (>10kb) Multiome | 591 | |
| chr11:64,304,032–64,307,072 | 21.7 kb | Distal (>10kb) Multiome | 994 | |
| chr11:64,316,954–64,319,418 | 34.3 kb | Distal (>10kb) Multiome | 1039 | |
| chr11:64,340,711–64,341,211 | 56.3 kb | Distal (>10kb) Multiome | 212 | |
| chr11:64,342,083–64,343,227 | 58.0 kb | Distal (>10kb) Multiome | 543 | |
| chr11:64,358,792–64,359,751 | 74.5 kb | Distal (>10kb) Multiome | 577 | |
| chr11:64,448,870–64,449,995 | 164.3 kb | Distal (>10kb) Multiome | 490 |
Genomic view of the BAD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.