The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Transcription factors with Perturb-seq knockdown data for AUP1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AUP1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AUP1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:74,261,293–74,261,813 | 268.1 kb | Distal (>10kb) Multiome | 184 | |
| chr2:74,374,253–74,375,246 | 155.0 kb | Distal (>10kb) Multiome | 158 | |
| chr2:74,379,653–74,380,438 | 149.6 kb | Distal (>10kb) Multiome | 229 | |
| chr2:74,391,129–74,392,484 | 137.8 kb | Distal (>10kb) Multiome | 876 | |
| chr2:74,421,273–74,422,464 | 108.0 kb | Distal (>10kb) Multiome | 756 | |
| chr2:74,426,172–74,426,842 | 103.1 kb | Distal (>10kb) Multiome | 88 | |
| chr2:74,440,246–74,442,723 | 87.5 kb | Distal (>10kb) Multiome | 1050 | |
| chr2:74,454,612–74,455,413 | 74.7 kb | Distal (>10kb) Multiome | 982 | |
| chr2:74,457,177–74,459,169 | 71.4 kb | Distal (>10kb) Multiome | 934 | |
| chr2:74,464,912–74,465,776 | 64.3 kb | Distal (>10kb) Multiome | 842 | |
| chr2:74,472,106–74,473,059 | 57.0 kb | Distal (>10kb) Multiome | 1071 | |
| chr2:74,481,955–74,483,673 | 46.5 kb | Distal (>10kb) Multiome | 1031 | |
| chr2:74,497,707–74,500,027 | 30.2 kb | Distal (>10kb) Multiome | 553 | |
| chr2:74,502,724–74,504,160 | 26.4 kb | Distal (>10kb) Multiome | 816 | |
| chr2:74,506,905–74,508,524 | 22.0 kb | Distal (>10kb) Multiome | 1063 | |
| chr2:74,515,477–74,516,725 | 13.3 kb | Distal (>10kb) Multiome | 262 | |
| chr2:74,526,271–74,526,489 | 3.2 kb | Proximal (<10kb) | 376 | |
| chr2:74,529,210–74,530,815 | 145 bp | At TSS Multiome | 988 | |
| chr2:74,548,706–74,549,795 | 19.8 kb | Distal (>10kb) Multiome | 625 | |
| chr2:74,553,035–74,555,636 | 24.8 kb | Distal (>10kb) Multiome | 768 | |
| chr2:74,647,804–74,648,489 | 118.5 kb | Distal (>10kb) Multiome | 232 | |
| chr2:74,653,808–74,654,908 | 124.5 kb | Distal (>10kb) Multiome | 658 | |
| chr2:74,715,073–74,715,890 | 185.7 kb | Distal (>10kb) Multiome | 768 | |
| chr2:74,777,656–74,779,073 | 248.9 kb | Distal (>10kb) Multiome | 426 | |
| chr2:74,816,374–74,817,201 | 287.1 kb | Distal (>10kb) Multiome | 373 | |
| chr2:74,822,732–74,823,334 | 293.3 kb | Distal (>10kb) Multiome | 40 |
Genomic view of the AUP1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.