The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Transcription factors with Perturb-seq knockdown data for ATG5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ATG5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ATG5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:106,039,823–106,040,588 | 285.6 kb | Distal (>10kb) Multiome | 109 | |
| chr6:106,080,045–106,081,482 | 244.9 kb | Distal (>10kb) Multiome | 121 | |
| chr6:106,085,814–106,086,566 | 239.5 kb | Distal (>10kb) Multiome | 502 | |
| chr6:106,102,889–106,103,993 | 222.6 kb | Distal (>10kb) Multiome | 277 | |
| chr6:106,154,774–106,155,732 | 170.5 kb | Distal (>10kb) Multiome | 203 | |
| chr6:106,325,124–106,326,565 | 107 bp | At TSS Multiome | 957 | |
| chr6:106,335,179–106,335,341 | 9.4 kb | Proximal (<10kb) | 104 | |
| chr6:106,360,205–106,361,449 | 34.8 kb | Distal (>10kb) Multiome | 616 | |
| chr6:106,422,406–106,423,164 | 97.1 kb | Distal (>10kb) Multiome | 102 | |
| chr6:106,511,736–106,513,208 | 186.7 kb | Distal (>10kb) Multiome | 661 |
Genomic view of the ATG5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.