ASAH1
N-acylsphingosine amidohydrolase 1 | AC, ACDase, FLJ21558, PHP32, ASAH

This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015]

Member of: DE-1 Developmental clusters: GC5
Biological processes 39 terms
N-acylsphingosine amidohydrolase activity (GO:0017040)N-acylsphingosine amidohydrolase activity (GO:0017040)N-acylsphingosine amidohydrolase activity (GO:0017040)N-acylsphingosine amidohydrolase activity (GO:0017040)cellular response to tumor necrosis factor (GO:0071356)cellular response to tumor necrosis factor (GO:0071356)ceramide biosynthetic process (GO:0046513)ceramide catabolic process (GO:0046514)ceramide catabolic process (GO:0046514)ceramide catabolic process (GO:0046514)cytoplasm (GO:0005737)early endosome (GO:0005769)endoplasmic reticulum (GO:0005783)extracellular exosome (GO:0070062)extracellular region (GO:0005576)extracellular region (GO:0005576)extracellular region (GO:0005576)fatty acid amide hydrolase activity (GO:0017064)fatty acid metabolic process (GO:0006631)ficolin-1-rich granule lumen (GO:1904813)hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides (GO:0016811)keratinocyte differentiation (GO:0030216)lysosomal lumen (GO:0043202)lysosome (GO:0005764)lysosome (GO:0005764)negative regulation of DNA-templated transcription (GO:0045892)nuclear receptor binding (GO:0016922)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)regulation of programmed necrotic cell death (GO:0062098)regulation of programmed necrotic cell death (GO:0062098)regulation of steroid biosynthetic process (GO:0050810)sphingolipid metabolic process (GO:0006665)sphingosine biosynthetic process (GO:0046512)sphingosine biosynthetic process (GO:0046512)sphingosine biosynthetic process (GO:0046512)tertiary granule lumen (GO:1904724)transcription corepressor activity (GO:0003714)
Expression (TPM)
ASAH1 — as a Regulated Gene

TFs regulating ASAH1 0 TFs

Transcription factors with Perturb-seq knockdown data for ASAH1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ASAH1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to ASAH1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ASAH1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr8:17,800,593–17,801,971 283.5 kb Distal (>10kb) Multiome 778
chr8:17,912,201–17,912,908 172.2 kb Distal (>10kb) Multiome 360
chr8:17,922,394–17,924,221 162.0 kb Distal (>10kb) Multiome 992
chr8:18,083,277–18,085,104 633 bp At TSS Multiome 1029

Genome Browser

Genomic view of the ASAH1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr8:17,790,593 – 18,095,104
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq