The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ARHGAP35. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGAP35 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGAP35, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:46,587,845–46,588,467 | 272.9 kb | Distal (>10kb) Multiome | 21 | |
| chr19:46,600,411–46,601,577 | 260.0 kb | Distal (>10kb) Multiome | 1006 | |
| chr19:46,633,826–46,635,061 | 226.6 kb | Distal (>10kb) Multiome | 365 | |
| chr19:46,638,437–46,639,929 | 222.0 kb | Distal (>10kb) Multiome | 741 | |
| chr19:46,659,987–46,662,280 | 199.7 kb | Distal (>10kb) Multiome | 691 | |
| chr19:46,696,773–46,698,048 | 163.5 kb | Distal (>10kb) Multiome | 408 | |
| chr19:46,713,710–46,714,718 | 146.9 kb | Distal (>10kb) Multiome | 566 | |
| chr19:46,716,676–46,717,696 | 143.8 kb | Distal (>10kb) Multiome | 487 | |
| chr19:46,745,601–46,746,962 | 114.6 kb | Distal (>10kb) Multiome | 561 | |
| chr19:46,787,215–46,789,013 | 72.3 kb | Distal (>10kb) Multiome | 944 | |
| chr19:46,850,211–46,851,336 | 10.1 kb | Distal (>10kb) Multiome | 946 | |
| chr19:46,855,920–46,856,711 | 4.7 kb | Proximal (<10kb) Multiome | 610 | |
| chr19:46,859,815–46,861,611 | 653 bp | At TSS Multiome | 1061 | |
| chr19:47,019,502–47,020,076 | 158.7 kb | Distal (>10kb) Multiome HiCAR | 188 | |
| chr19:47,035,599–47,036,293 | 175.0 kb | Distal (>10kb) Multiome | 627 | |
| chr19:47,047,837–47,048,980 | 186.9 kb | Distal (>10kb) Multiome | 760 | |
| chr19:47,058,561–47,059,308 | 197.9 kb | Distal (>10kb) Multiome | 357 | |
| chr19:47,111,821–47,113,936 | 251.2 kb | Distal (>10kb) Multiome | 1117 | |
| chr19:47,130,439–47,131,651 | 269.8 kb | Distal (>10kb) Multiome | 908 | |
| chr19:47,157,325–47,158,494 | 296.6 kb | Distal (>10kb) Multiome | 271 |
Genomic view of the ARHGAP35 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.