ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ARF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:227,787,209–227,789,037 | 294.5 kb | Distal (>10kb) Multiome | 579 | |
| chr1:227,946,348–227,948,809 | 136.0 kb | Distal (>10kb) Multiome | 645 | |
| chr1:228,004,351–228,004,883 | 78.1 kb | Distal (>10kb) Multiome HiCAR | 154 | |
| chr1:228,058,680–228,061,126 | 22.9 kb | Distal (>10kb) Multiome | 377 | |
| chr1:228,073,942–228,074,172 | 8.5 kb | Proximal (<10kb) | 116 | |
| chr1:228,081,634–228,083,952 | 98 bp | At TSS Multiome | 942 | |
| chr1:228,085,140–228,085,832 | 2.8 kb | Proximal (<10kb) Multiome | 276 | |
| chr1:228,101,882–228,103,743 | 19.5 kb | Distal (>10kb) Multiome | 929 | |
| chr1:228,108,924–228,110,105 | 26.7 kb | Distal (>10kb) Multiome | 958 | |
| chr1:228,130,684–228,131,457 | 48.4 kb | Distal (>10kb) Multiome | 488 | |
| chr1:228,139,579–228,140,870 | 57.4 kb | Distal (>10kb) Multiome | 931 | |
| chr1:228,164,898–228,166,840 | 83.1 kb | Distal (>10kb) Multiome | 1011 | |
| chr1:228,174,477–228,174,893 | 91.9 kb | Distal (>10kb) Multiome | 254 | |
| chr1:228,212,247–228,214,390 | 131.0 kb | Distal (>10kb) Multiome | 604 | |
| chr1:228,275,524–228,276,595 | 193.4 kb | Distal (>10kb) Multiome | 428 | |
| chr1:228,378,133–228,379,597 | 295.9 kb | Distal (>10kb) Multiome | 515 |
Genomic view of the ARF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.