Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for APRT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = APRT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of APRT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:88,533,022–88,535,493 | 277.9 kb | Distal (>10kb) Multiome | 458 | |
| chr16:88,569,589–88,571,104 | 241.6 kb | Distal (>10kb) Multiome | 904 | |
| chr16:88,634,178–88,635,155 | 177.3 kb | Distal (>10kb) Multiome | 384 | |
| chr16:88,650,385–88,651,684 | 160.8 kb | Distal (>10kb) Multiome | 890 | |
| chr16:88,662,399–88,664,138 | 148.7 kb | Distal (>10kb) Multiome | 943 | |
| chr16:88,686,082–88,687,017 | 125.4 kb | Distal (>10kb) Multiome | 675 | |
| chr16:88,705,859–88,706,820 | 105.5 kb | Distal (>10kb) Multiome | 838 | |
| chr16:88,737,237–88,737,815 | 74.4 kb | Distal (>10kb) Multiome | 619 | |
| chr16:88,769,688–88,770,137 | 42.1 kb | Distal (>10kb) Multiome | 282 | |
| chr16:88,770,700–88,771,325 | 40.8 kb | Distal (>10kb) Multiome | 668 | |
| chr16:88,784,542–88,785,880 | 26.6 kb | Distal (>10kb) Multiome | 811 | |
| chr16:88,802,766–88,805,068 | 8.3 kb | Proximal (<10kb) Multiome | 881 | |
| chr16:88,810,968–88,812,892 | 116 bp | At TSS Multiome | 1059 | |
| chr16:88,816,092–88,816,286 | 4.2 kb | Proximal (<10kb) | 278 | |
| chr16:88,856,080–88,858,003 | 45.1 kb | Distal (>10kb) Multiome | 885 | |
| chr16:88,940,964–88,942,110 | 130.0 kb | Distal (>10kb) Multiome | 323 | |
| chr16:89,093,591–89,094,129 | 281.9 kb | Distal (>10kb) Multiome | 694 | |
| chr16:89,167,237–89,167,783 | 355.7 kb | Distal (>10kb) Multiome HiCAR | 740 | |
| chr16:89,170,902–89,172,416 | 359.6 kb | Distal (>10kb) Multiome HiCAR | 464 | |
| chr16:89,217,337–89,217,960 | 405.8 kb | Distal (>10kb) Multiome HiCAR | 857 |
Genomic view of the APRT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.